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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=W20p1YLjxWZBxbnIsasw17EOd7Sf4d8nAzmD5-KYoia5ADfw60B4bL2v4MIG4PTeEkK4Zg9NADDXJlquJ8EBBX62C7LkkHImolJPLnLTLNHMnncTs-ly0wQa2kv2L1uaRHTI6jHBdVy925FgZCurD1Pc7Gkm6tDTeiXTiiMXiwW70bWFR0b7t3mNTC7XbRy-LWHGHR9xUE8fqjBWZFl1qVdsWgtNAcfqVibnJCmSbErcbaFMZkmbvA327P1EpUM7eM0vxe3xxzJc11ZXDXEmasHnKFLJ9XZeta07F96bdLIOclQAnhhumu2f8pWNUeprg24U9wh8hZPOb~qG0ip5xg__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=DJlO7eNeyXUZPPETr4tnH2w5B2Yl5jGdmZLGVr7CRGewe~yDwlD0yWCKbYFyAxEcbRl-JE7paCSV~qaTBiOzcrgyQVf42KtunCGdMeBSJoBHr0CijINKZzlc8P9GC147l4Mqym~~vO6UgTReG6MNCSI5CnOSl9islA-NVEFKzwie~WNGKeZq-xKW2sKmqCr4mBi~iY2OyLtE1oXUmDD-lXkJ~N1jJ8VItyPRpRM-C9gLVqhMFJrvX827FH33qXlskjwo-To1Jw7LM0sP1ynskr6HkahB2vGfnPfSrvndfhp1kg3xYuxjMyrH6wkL9PsfIp2e2gSzKCxP1ywGOFEjpw__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=fHdQ9TQR7iCKn70xOV-x843qaB4phiAhSET-YFU6l5OHGBQo~1j2~K1g3e9gw6u8Rp2A9QJAbw39KGhUax5bT6YPUJvI-eFc~FHW-kKgbKnolb4scHUA2RAZzwPilKX2OR5vflAiYllQqkpErzApCrt3CK0ZM4~aK8sLCrruiI9y8LGYM90szgZ7mq-mqLw0U51D-nnXI-S45bFp7ddU4lIDmBZD2pmpRy4H56Gk8T~~uFCNmSnCLG27R7ehryX8hxC30RAzf60m6ElOOVCoXxY9T8TTQAtoJdsVnm70yji91AxgPxS8HMpH0x2CaNas~62065u3eaNwKikJET1vTg__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=iaiK3R3YS7PRR2sEqjPVdfGAyO0KU4HmMxuPdZRI-4CIdZZbHco~c1ZL3CiKWJN19n2rHm0q50~olk5EJxhWuX1BSQC8~TcFKtm6nGqIvyWbIzkgiw12gpshSwcncOvOb6YOugrAzgvCfDSYveAzQxx8Bw-XN1m2ywBkS0RV-UuVSPhgAWFOrMIh7kTcOq1Ptjnv1KKhaqemyVDOcQGZzyP9rGIfzNhWMoxKAS-xGZN0bA1VOJM0a4JT4qrepj0j0gVClCCwhbmV7lPnttgRcQPZYLdCKT2wW9iBGQu0Skzr~hV-vp4x3hx~aZ2MpjomNddGOzwQkjt0cUVuD49FbA__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=RVJGXwitU9JQ-uNzwJYhfyyup1ALh9hp5OmZBbYAuBb4U-pLK3616Zqwn~0q5EIcdDEadTCNL9LfFLvU~2do1ZcXh7zgBDFGKBosf95ZtPt4sOgCCf-~QKnUwataEX47TeoY3yIJI5b4OKNlR2XtOsehxnMUK-xYkV0E4RxKPQTsUZUvUVfr9PHV79gq5y5lYLwYjzMGXUrnYknMoV61dlnBwAFD~NSPMhE7HIfBL-woiCwESASwHz8P7ZgSG4QZxIJKJh4DKnXOsZu1FAy2F2geEZP5gFXdKNZtkUvY5VVy~fMZsAtaJl~MdBW9tTmAB5xUjU7sNXVqKh8heDhJPQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=F4lIYMsdEoOEYAd6tzqLhSpeLY6ms9sMrtJAIg~kXhePsYQJjUl7SQmuCAVoCkgCf8gC~VhY48YGbrgMAZBfJItrgxKBhg4sViAmsHnOYHrq6DIz-juoSTpPrSqIR7QmWF7V7QXbBfyV70lPhX96sOeNJlmcRJX7jxIgqiKcHQkPRds2U~L4KUedIUkOl67N8Gr7c6~yOoeNR-Yrwb24PBoVo3h5kZcCvz6y3Ur7YgVqcuYCVojbHr0OVHovwRAPLtf53FZjMnqKB7~pczj3CnFdixy~bd3bCNFUECTgfOSTV113-AFMJAIxPOMyCgUD~kv1082-fkpmr0-Cecn6mg__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=BTfByIufdGoEaLtID3PAL0iApbngatT5QRPw7RS7oL7E4H-7zjCnYEXV7FpJae1D7ug8ZY9rhl1ojn60fQs~-EWqV5BykkvE9DrqeMlAd5E53WDfOArUzVYVsIVjP~C4DAfNxWYSqP~Qg52LpM~s3~Ae24x8rzzDopyKgqpMNyc3EcJTiKKlyb-OXwPFprV2~XcNTa3~CFCfYKoBHnfqI04qV7bWznEC~llB1zJSu52FcOVwAHyVDRyrYnV0nnosBqek99E1KQuT-E0j1GxVQLuugkHqUNVzCOvb9dUeBM-6kL7ZpDeRe6UtpRIipw4dFrGT3l7Hco-xKWfnQxQ1YA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=NmLacxF02o9m9S23NVkSC~gKBEpVKzxPNZ1cbl1OxMEd5C5Mf6VV95oEu4bbN~xcCRiZNRp3rGWwf3EvR4QR8DQf2tnWVkW7mRm1mit0Xiq-3k1ZGe8xL3r0XkqwYqK7VbOzEnhgBpSKu7S4GvHbWbF77i0SHKdvoez78CViJaj0wn2Wdylfkmme8Ezcd5gCX7NzfACfSkVKUOyVY~7HiEMPVsAP9iikMGH7P5OreXa4zG5Kknc93zLGG8ZN8yNnfxkWHwhGY2bFFN1bPQn~pki1lUGwxVLkt0ACkmJZygf7imqdcqdxE56uJLQXuMWN8XmRmw3RKI9X0jtLl-1Zzg__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=QfLP8nmsXIjLCNXl4iBcN9Xn0uvjS69ulhJNyN8EarqQi9nDIhCcLdIGy-tR4ieRAipb4VO2ads6ltlkL2N9wan-K4rlQQhXEQliPvqJSJn9Ou-Iz1U4j7Yc9S6czv~U5EcwLbvHJw7lWfxAVNTI4vWbL~NORnq-S5qEwTh3kYe95n1IMdlVsTfxR6sIXGaOwFl6N2JAbnex67mkSpfBWDNN6EhfxASakmb1v3q0RjKw~daAgRQsWeSZd7BOmj~5Y6dQLZIQ3SlvIIihdTtO-3yYF2xa7G0FpHeJe2~dyybL5Py8YMI5-XUNd5dbwBHFnciqDJDBRUY6gEyZdvl48g__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=E6~4DwVw1taKYg~-Pdla0vTwrn5JBLTgUqcnlzFm7Cw8uxWO~zrgJJPn7XmN1P9iK5-Z4qBTt2DQxKOqBvyP-jGVXgjDSP~-ZEL-NFukLBVohGRR8f6zw4olWFNyrRp3FoPA0eR5I~8moY9YYcr9-xUDhAKj8wyezIePqXLoKkaeMEs9dpTzBf1YpreD0CYw-S99YF~~vmhyCJb9EhWovyRaHwvtEewFTQ0CpJg4LdwP~jflIMCpQobKKF2MdSNzGBmP48jr0TXbwdxh1vvULqDolJ7LooGwsfowFpeYf~fmS8tZkl6GsIc65ts6wixAoiNqbHLw5cYmWuO3iO1erQ__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=HU7ycJZ72CKMfPeUvoeM~4igpbPwHtKdtaGFsC1rGHz~HivXcCcjLP6fC3gPpoLrm6F~Nu~A3HMGB7RBfEzeeS9AJx~DlgV9Q4NRqC0g-~pR6oCuRmAiyTrQ10zJm3d5IAwJEhOfXRzd79ZoFpo4Ffwys3sVDQTYEP09sLdAU-say3IizUh-~0~VaFNQWD-INv7CghfgbYLZpAzhEv9szZpUxn91S6sRYkYZF7~UnS0SIuZtE3zevmIXuh3CNzVK0oK1GSiIuauK-W7SrFTGzJZZZGWhvFGMbgoi8ESij8rw0GPfLeJzTSfZaPQJX-azQZaju0nOiyVvQp8UfIsG4g__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=AHWaQ6xqCfTFyscfYe8WWXfA6xeXvpRelX3UihdwTlEb-bD6ssHaJHuSeKtd6QkaQaRxAQVIr5JJHjkYx75wQuGh7F3yAXiNhUiC0khjd-Xhqdfj-set8Iz6DhKG3GhtbHKpVvEar1hjc5JIjD1UoDGAczgrvL1iAjMSAordJ3wU09AS~vKre7wNCXonvhfTDXh-ctuRHQHvsXLuxxX979FzGd1jundTuSKFaBUjddyic1zM3-Dp7zYi78hmGG1FhGUpbv-EphnvjGc9IsC2GONiojP5Rq09uNkQQcRm9ZlNaDwXQqe~-WAYGycV4ZzPZGu~fy9l~hPV4DyARv91pg__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ow9rxf8KN2wRn4UaAQ5sjNviFAoj~wYnHcZ5uTMegU3dzk3un7q4S1zQg3P1VAzqaxz8~AOOvHQ9hRUrG-xIuZT6I~aN3bcyHxXiPD1hnMnXK56P7TJUrGk3wXdQAtTUPPz-tFgBb1nPtBtj3HLAcNIzaeTX02XSxyPpA387exvf0Kr6aqBG5xgvMhLZ7avtM7-fuRS3oTMNI9UPE2qmzFnIFg65zsb1dpgEL6wJd9CRgTLa8uVOnTGb0zL3EkPtUjjaiE2iiB9l909YjpvW6fQ2iGGOb4W9F-da~JgL6YItKO5g40KlmHjnZsL46bWwAzGHG2Qx-7CVQdPDl3W0xg__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=hwmdvOzHbXM-fFeiNTY22XZwyFpKem~ZTWTx6PqiGzckcHTqBL1hQ76iy-2abwYneMiYs10Yj-3TfoYIcZADRRAphyDniQpcKiGEndQgZeca1YMlTT4w6qh51N-Ry8wscjFBZ~exGQAA~P6wt-LkXMgINXD59R0re7Ld2CsI6Lkt51Vx3OAOrQ5ChJoZkC7aY70P3BbyG-jlmMDKw8XkmLhVv-rEoaKtyb~9UprG0rqqHQq7aSq58NdwKF3r3ogWdzr4QSVB4PeCqSeAPxCc03lMkoTV9BsMNTTR6E3loizBXQ3kfOAaZVNh22rG5wsuIpUjY5YuLUsEeBvcxt124A__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=NmeGiqscH8rHqFqCyY-x2M20kyH~Khu0JS9CCrrVpsXvDcKkkKc1ZDw2xHx2gx02weBprLGrVS6WH00g~t5ny87tfGRozPgKfLBeLZ~LJaC7dP8CFPo5NS8vCHs3XVHgs1G3kXUKUyWwmX8pHMxkR~1Mbyec3dKBJwL2i-Pxa4GxferKyWEsmzutjti8~vG1SmOuOAjaKbXxHuqQ5I9U1w22yQMlRz-42b-5I9hdVk5II7aJCxYjk-H6qGUHZ0j~f8~RXCBDUwMWW0G8IqOn3F834YjpOLNFU7O~mKunrOnsQnWCxJiEGobBlSU455819wAHCwFKYi~C2V6L~F2aCw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=n2uQXFK3qWVZeHMcMxJG6q2J0~OjDeFx-7SE1iEZ~pF7bKAUJDp7MSz68HwvExuaHy3BPTwL7dknEgz71J0vbsTi~fWNSLjKtEHsIN1d8vFSONC6EF1Rp5Mp877WrZRWeTp-NjSPYIGQCgULhLnqVjG91yXWogRELHLgr1khZWlp4QhMDil7LAiXZ~MsCgZ1Epcbp-ZrJMAFjy303mgB6lRsPwUmvaHDEH4EjT66WhuGT4XAajyATuaCiEKirlhn5NG48IYWpnyG1N76-aPb85jtDrOoF5YyDIN1A8G6Ol9aHNlcIVO2MGZfvV4NT5p-jSsju0Uu~sRlh-qzPX1tPw__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Hj2~RoVg57Ff0ptLI8NqDxZAmyQqqvDtrMQyztVuIA5aIwIU8VTS~~-2l8XOSE3HGUliwWIJ0Ry1RxVKyLpXCKqTGO3GQtwulz~tYksDC9L2n6E-47nEnaZUypoRDmWeeKI0pXoFKtXjp1NhvSV0GBv5g77eOPnLEBl0GuB2Apqap9eV10dvyzl-2MPSZ9IpMh3Ks8-Z6aAg-8abQuoH6sYRea~5UvZNHR7REyMbTQMhqt9uKGh6HgAmCb7NK423YlDj84k~UMBIph9LcTnTaxvKQVfBpEKJL4gYVVmufm2R2XplPdt-wcRVwZoJ8-qgE54YdtUhvyhDTyq4RGbveA__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=XRh95IY5mNf4zO5nUShuycFM6l4ntFT5XIA9OlHUdk0qZv53Kb5zXXwBAJDP068DyZHZ21SZO8GpF6566UJh3IzhYhSP7M1D3WeEnQLPu-HnK0EwRjnu1QpbZuYzRBvmyH3z4GiwOq4DtxAy5hNwXx2Lt52vdH9iwmuqwor~Uh0gzVpFkdXTnrdAEvOhUxSpMv7vJMQ-6bXM2z9VRwM6faZgHfEfaL84huVp8WlgtR1-ROD~SbfR-p0cKjvecpJlFLhJIdakZzZAOKdLZ333jg9Tm2rd0Id8bn830zur~vLLMtJt~AFo2VUafP1POA2z8Q9MS7q5vTVgxbG~ThOP9A__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ThZO-0ZFtIaa6OobqJ457-ShXk1J9m2zAoPOnZA194--uV632ckAWfm~GTdT7VJA3rZHiPOZwx0rlppJmoXXdAEYO8tsmVeqwBkiIotDYZs7FOjggJii4nNhH2PDJupQ34BjMkkdNaiQuhlIraaUSR3XzyPhNl-KOHzZJ9KpvV2ULzMmHihFdfwkzaI256L~INeAb2gy19o9CnIxT9~Gx4Ex6WxCGhEYlONSsWkTd904TVwCN6U5uY0aiQx4jMe8G~zUxCwZ0J780ncv~95Wvbgxwh8gGOV--vqUyzh6W4EXIfbnBoYej7oXY9JVRgY5X~JT9ABJPjkB-48aQtTgmQ__"

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Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=V4PdYEmqRCG52cBHwVD6581Y6cROutsZjP33bL-vhr5XWgLCcBNKouBaIoRsXAB5iUIkK1pA6Zw7mUmUp-soh9E4Y6WTouxatNRzJcf9mMscjC4FNnA0i18rG~8dg2xsj3xS2VP0tw4iviMJB6wcL8zc1gYUXHtdoSEGuvKVAiXt-hLQsqA-t0MjcBoGIvh6HrVd3j4KhiYsyg5FL~BqfyiH6kO4x4DL5dnuhqSbYuenAVwZTXsA9-rL0eZPoS8i3K3Fu905a48~4m3RPVn0p6DgPD-26nDrNs-Rd9xIB4CHvATNIX7b6sI1p6KWpOvKf7lwXeHCw6NUR8C8Foik3A__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=MQR7KhSI6g2xyjCixKF8I7PwEE~OwHp9FdtTozgtYwiDH4NFpGL6cBj84376eWNO0I4YmiTUweWHjiicoSXpCk0uTrKrsJwhKL5IzkG9WNWwMtxWckkn3y1~uIq1cmdI7gsn7chGxOnRSF4YSZMit38yLk7MXWy2y3lXunbS6yYdYtp-dapKasUagWUxOYfZV~w8oRKdA7VyEe43ZgSn3FrkFHGbkCBeLV5n4o~JhTVt4ciE2urFPrIGWW6IvuyqszNkFulKVSsxUBl6JCoQAzw6qBeBnpTtiO33fEWxglwa6LBDcrEwoDB4WAcPNhj7mWQsT34BaWZXllpXSAeSMw__"

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Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=VyKOLOg~WJXecybou5iQtEcdymkU0Ckn8tCJO46YLLWNdUjqranf5pKUuwqPFabBYbVL-qd6ULMKt37y0PzBFoT0Wr9blcp0DG1U07uyzNl2XKIPVDQRS~TOkl1BY2DRvCbowppFgxePNoo55zNOvLKSNQ188vOd6ilIx2qk6k1jPi4IdyForKNM0XGT~BjNc1aoV7AqndOvT362fC~HurXbOxYYCsrr2-LHTk-fgGUHFsj6wuVVmDV1S5TL3gUN0sQwQXOvb8~qszH64Eif5j45MpQ-WLZxjc3W8yFOvLsm~3~gCdBd4kfxcFL~EgduNBqMn7QKrBZwGG12Gs3nzA__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=RgRcfRJuUF-C0BKgsU2E1SD5PbdnsLRQSlGrgu82BWDW6Ujd37c7WCgCLhmPhtCymRDs97MWs6iKrII4Q6jtlR4pwgu69Q4nP7Ib1-bioo43diTXwyNmHqbd-hmyfqW7svgO4b-QkOxs2HIpunmDk-k-cDME13oE5ENIbuyqZqcI1rjLtOk2MQUXrv~x3~lkqQhCrpv~Bre55yHL-vZmCrTDOF7aQW4K9zz~h6Ca0pgbNgvwea0rPm3cWrzv8IWcj92oF5wEXLuJdn~LHNMQuTQFZPpSj9EaDwCw9ky9WDAcsaGuHfPT6uQVdyrR62KjTjjIUTgUxdOoIYrMd5I8kQ__"

Download is unavailable on mobile devices.

Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=dk1kcYao5JnxBei5s6vLQyDeZ-yDrK2hF7iP7BhpwQBRUEQlsoI73qR89ARKojNOuvwFEJLvkxdfkPV-CA3IR3DGc~3LiLvxBB4DWAoPgZqCPs4yWWSg6wi6JK1y27a9OsB5ExRyHnsPP4iyZZS~GsscAj02pJ-u9zDP6YQJGRHrcXrGb7z4F6w9h7psRGm7Qgly5DO1YZjtheBy1~KxEx8Rxaaio28jKup0b3AgpskWnGh4fxlx~FgOqphcVMykl-AZuawZ3~KTD~SuLdHy~DyiujGF8quYVk9bOwEwOZexugHy8BRnTe33VEJ9DFgnHm9rx69~tX7gvMabggaFXg__"

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Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1774638792&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=l~tStWTfECh9bRzFY95oMFxqLZ0yc-MFQ1ke4A7tjkPkeoHd93nJs1Uf4vmb8vOk46aPdLQO6TZdWShMGeYX3~dzZM2NbZBIwXdvZP2lWxgqH7woavryYqnwbiZHxgX5ju2eFYr5ZXAjEIzZ8ipl9Z1gnPMO0PMKZmQ4tHmSZdSD3YI7Z5ge6eB6IrP6M3wh9~Ki27RbvvJAGYqV3YLTtx227a8KF-cMRo6xV7EhjGjuBVCnna1xGqWQX7aBsfs4lTBwIsyFfM1P7W0EuigW-9~yMpdru1p5JZ5NAzh13Jf5xQrSSHvoOw5bJW0tDNDgxFviORfQTH8Xe-RJ6LVNQQ__"

Download is unavailable on mobile devices.

Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.